Isolation of Cancer Epithelial Cells from Mouse Mammary Tumors.
نویسندگان
چکیده
The isolation of cancer epithelial cells from mouse mammary tumor is accomplished by digestion of the solid tumor. Red blood cells and other contaminates are removed using several washing techniques such that primary epithelial cells can further enriched. This procedure yields primary tumor cells that can be used for in vitro tissue culture, fluorescence-activated cell sorting (FACS) and a wide variety of other experiments (Lo et al., 2012).
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عنوان ژورنال:
- Bio-protocol
دوره 3 3 شماره
صفحات -
تاریخ انتشار 2013